Microbial spoilage of Some meat Luncheon Samples Collected From Damietta City, Egypt
The microbiological examination of local meat luncheon samples obtained from local market of Damietta governorate was studied. These samples were belong to two different companies in Egypt. Bacterial counts of luncheon samples were 2500 and 310(×102 cfu/g); Bacillus cerueswere 470 and 30 (×102cfu/g); Staphylococcicounts were 170 and 30 (×102 cfu/g); fungi counts were 30 and 1 (×102cfu/g) in company A and B, respectively. The high level of Aeromonas sp. in luncheon was in company A, while the lowest value was in company B. In company B, coliform bacterial count and anaerobic bacterial were 10 and 450 (×102cfu/g), respectively, and they not detected in company A. Listeria sp.; Salmonella and Shigellaspp. were not detected in both company. Total bacterial count, Staphylococcicount and anaerobic bacterial count were higher than the permissible limit of the Egyptian Organization for Standardization and Quality (EOSQ) of Luncheon No 1114. On the other hand, Salmonella and Shigella andcoliform count were in the permissible limit of the EOSQ. Fourteen microbial isolates were obtained from Luncheon samples, six isolates from company A and eight isolates from another company B. Three isolates obtained for each Nutrient agar and PDA media. Two isolates obtained for each selective agar base; Baird-Parker agar and m-Aeromonas selective agar media. One isolate obtained form EMB agar and cooked meat Media. No isolates obtained from Oxford Listeria agar base and SS agar. Among of eleven bacterial isolates, six isolates were long rods, three isolates were short rods and two isolates were coccoid shaped cells. Six isolates were Gram positive and five isolates were Gram negative. Seven isolates were non spore forming bacteria and four isolates were spore formers. All obtained isolates gave negative results in acid fast stain. Moreover, Rhizopus oryzae, Penicillium aurantiogriseum and Cladosporium herbarum were isolated and identified from Luncheon samples.