Chemical and Microbiological Examinations of Water and Fish Takenfrom Brackish Water of Damietta City, Egypt
Brackish water and fish samples of River Nile in Damietta city were examined. Morphological examination of fish obtained from brackish water of River Nile was examined. The highest value of BOD5 20 was found during summer being 17.4 mgO2/L while the lowest value was found during winter being 9.9 mgO2/L. Stannum and arsines did not present in all examined seasons of all samples (water and fish). Lead did not detected in all seasons samples except in spring being 0.003 and 0.003 ppm in water and fish, respectively. The highest value of total bacterial count in brackish water was found during summer being 4300cfu/ml x 103, while the lowest value was in winter being 2 cfu/ml x 103. The highest value of total fungal count was in summer being 18 while lowest value was in the winter being 0.8cfu/ml x 103. There was no bacterial growth on SS agar medium. The highest value of Staphylococci count was in summer being 30cfu/ml x 103. The highest value of Aeromonas count in summer is 50cfu/ml x 103. The highest value of coliform count was in spring being 150cfu/ml x 103. The total bacterial count of fish muscles were 9, 1600, 0.0 and 0.0cfu/ml x 103 in spring, summer, autumn and winter, respectively. On the other hand, the highest value of total bacterial count of fish intestine was 3000 cfu/ml x 103 in spring, but the lowest value was in the autumn being 0.1cfu/ml x 103. Total bacterial count of fish surface was 390, 1000, 0.1 and 1cfu/ml x 103 in spring, summer, autumn and winter, respectively. The highest values of fungal count in muscles, intestine and fish surface were during summer, spring, being 1600, 3000 and 1000 cfu/g x 103, respectively. Ahigh correlation coefficient value (r = 0.747) between the log of total bacterial count (LTBC) and BOD in the brackish water of River Nile was indicated, which means there are a highly pollution in river ecosystem by industrial pollutants. Among 18 bacterial isolates, only 4 were coccoid shape, 5 isolates were short rods and 9 isolates were long rods. 5 isolates were Gram negative and 13 isolates were Gram positive. 9 isolates were spore formers and 9 isolates were non spore formers. All isolates gave negative results in acid fast stain. Staphylococcus sp., Micrococcus sp., Bacillus sp., Esherichia sp. Aspergillusglaucus, A. niger and A. flavus were isolated and identified from water and fish.
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