High external Na+, but not K+, stimulates the growth of Ulva lactuca (L.) via induction of the plasma membrane ATPases and achievement of K+/Na+ homeostasis

This study aims at investigating the specific ion effects of Na⁺ and K⁺ on Ulva lactuca (L.) growth. U. lactuca was grown in balanced nutrient solutions with 10, 100, 300 and 600 mM NaCl or KCl. The growth was significantly higher at 300 and 600 mM NaCl compared to KCl, with the highest growth rate at 300 mM NaCl. NaCl-treated alga showed increases in the photosynthetic pigments and Rubisco protein content. However, KCl treatments adversely affected these photosynthetic attributes. U. lactuca needs adjusted, but not high K⁺/Na⁺ ratio for a proper growth, since the high K⁺/Na⁺ ratio in KCl-treated alga was associated with growth retardation. The cell wall was more extensible at high concentrations of NaCl compared to KCl. Therefore, the deleterious effect of K⁺ could be mainly on the cell wall and hence inhibiting the growth and perhaps the vitality of the whole cell. The transcript of plasma membrane (PM) H⁺-ATPase was detected only at 300 and 600 mM NaCl, implying that this gene was specifically induced by high concentrations of Na⁺ but not K⁺. The transcript of PM-Na⁺/K⁺-ATPase-like exhibited no Na⁺ specificity and its induction alone could not improve the growth of KCl-treated U. lactuca. The simultaneous induction of the two PM-ATPases could positively affect the algal growth at high NaCl concentrations by maintaining the proper cellular K⁺/Na⁺ ratio. Also, both PM-ATPases might contribute to energizing the plasma membrane and thereby promoting the cellular growth of U. lactuca but at high Na⁺, but not K⁺, concentrations.