The ameliorative effects of a novel combination of Taurine with Sulfamethoxazole against Carbon tetrachloride hepatotoxicity in mice: by modulating the antioxidants system

Background: This present study investigated the effect of each Taurine (TAU) and Sulfamethoxazole (SMX), either separately or together (TAU+SMX) against liver damage induced by carbon tetrachloride (CCl4). The research design was experimentally where one hundred and thirty-five N=135 Swiss male adult albino mice were divided into 9 groups: negative control (olive oil-treated), toxin control (CCl4 treated), Dimethyl sulfoxide (DMSO) control, TAU control, SMX control, TAU+SMX control, (CCl4+TAU), (CCl4+SMX) and (CCl4+TAU+SMX). Results: The carbon tetrachloride (CCl4) amplified the oxidative stress (by increasing Malondialdehyde (MDA) and Nitric oxide (NO) levels and reducing Superoxide dismutase (SOD), reduced glutathione (GSH), Glutathione reductase (GR) and Total antioxidant capacity (TAC)), moreover, the liver damage indicators were increased (alanine transaminase ALT and aspartate transaminase AST) and liver proteins were reduced (serum albumin and total protein). Additionally, white blood cell levels (WBCs) were increased, while platelets (PLT), red blood cells (RBCs), hemoglobin (HGB) and hematocrit (HCT) levels were reduced. Also, SDS-PAGE electrophoresis showed a decrease in protein synthesis. The liver tissue histopathological analyses confirmed the former biochemical results. Both TAU and SMX post-treatments were efficiently able to discharge the detected differences in the indicators to nearly normal levels. Conclusion: In conclusion, the order of efficiency towards ameliorating the hepatotoxicity effect of CCl4 was TAU > (TAU+SMX) > SMX.