Contamination of soil from crude oil spill is a major pollution challenge in Nigeria’s Niger Delta region. In order to detect this impact, soil arthropods were extracted using BerleseTullgren Funnel (dynamic) method from three locations of different ages of crude oil spill incident sites in comparison with natural controlled locality in part of Niger Delta, Nigeria for a period of two successive years. In total, 39 different species (15,558 individuals) were identified throughout the study period at the studied sites. Acarina (Oribatida) and Collembola ranked the major groups among the seven identified orders in the number of families, species and abundance. The overall patterns of diversity of the studied sites showed significantly different in species diversity, richness, evenness, dominance and abundance. Extremely significant differences were detected among the study sites in species diversity, richness, evenness, dominance and abundance. Moreover, the spatial variation among the study sites established negative impact on the soil arthropods; where the controlled locality recorded the highest account of species and individuals in comparison to the polluted sites. Meanwhile, the ages of occurrence of spill detected was an important factor in reflecting the diversity; where the short term exposed site after the pollution (Site B, <1 year after pollution) recorded the least species and individuals (species richness and abundance) and also the site with the highest value in species dominance, evenness, and diversity in comparison with the natural controlled Site A and other long term exposed sites after pollution occurrence (Site C; 1-3 years after pollution and Site D; 3-5 years after pollution). This followed confirmation by results of the spatial variation of diversity between varied soil depths and similarity measure. The overall diversity of soil arthropods demonstrates the negative feedback of crude oil spillage on species composition. This negative feedback diminishes with increase in the age of crude oil spill occurrence.