- Immobilization and characterization of Streptomyces griseorubens xylanase

Streptomyces griseorubensxylanase was immobilized on various carriers by different methods of immobilization, including Sephadex G-50, chitosan and Ca alginate. In all cases, optimum pH of the immobilized xylanase were shifted to lower pH than those of free enzyme. The optimum reaction temperatures of immobilized xylanase on Sephadex G-50 and chitosan were shifted from 50-60 oC to 30 oC, whereas that of Ca alginate doesn't change. The half life time of immobilized xylanase on chitosan and Ca alginate was higher than those of native enzyme at 60 oC. The Km and Vmax values of immobilized xylanase were changed irregularly from those of native xylanase. A packed bead column type reactor of Ca alginate immobilized xylanase was prepared under optimum condition for continuous hydrolysis of xylan, rice straw and wheat straw. The operational stability of the reactor was evaluated successfully for five and half hours