الانزيمات والهرمونات (303 ك ح)

Theoretical part (4h/w):
Nomenclature & classification of enzymes , Enzymes active sites & catalytic cycle of enzymes, Lock
& key Model, Induced – Fit Model of enzyme action, Factors affecting enzyme activity (Temperature,
pH, Enzyme concentration, Substrate concentration & Inhibitors) , An introduction to Enzyme
kinetics. Introduction to endocrinology, Sites of hormone production, Mechanisms of Hormonal action
& cell signaling, Pituitary gland hormones (Hormones of Anterior lobe of Pituitary gland) , hormones
of middle & posterior lobes of pituitary gland , thyroid gland hormones, hypothyroidism &
hyperthyroidism , parathyroid gland hormones, hypoparathyroidism& hyperparathyroidism , pancreatic
gland hormones , gastric hormones & gonadal hormones & placental hormones.
Practical Part
introduction and classification of enzymes.
Relationship between the enzymatic activity and temperature, concentration , pH of the reaction.
Rennin action on milk clotting ,specificity of enzyme action ( relative and absolute substrate specificty
.
Estimation of alkaline phosphatase, acid phosphatase in serum.
Non-competitive inhibition of catalase in blood, estimation of α-amylase activity in serum, saliva
amylase digesion of starch and the achromic point , factors affecting salivary amylase ,principle of
hormonal assay, revision.