Naturalizing activity and safety of human monoclonal antibodies against of hepatitis C virus

Assessment of neutralizing activity of the human monoclonal antibodies against HCV
and also study its safety in experimental small animals (Swiss mice). MATERIALS AND
METHODS: Assessment of neutralizing activity of the human monoclonal antibodies against
HCV envelope regions (E1, E2) by two methods (by HCV cc infectious system and by using
positive HCV positive serum as source of HCV particles (neutralizing assay 2). Dot ELISA
were used to study the activity of the generated antibodies. We tested the safety and toxicity
of the generated human antibodies by assessment the changes in biochemistry of liver
function tests and changes in kidney function test, Complete blood counts (CBC) and study
the pathological changes with different concentration of purified human antibodies.
RESULTS: Human Abs# 5 & 11 showed neutralizing activity by (neutralizing assay 2)